CYTOLOGY SUBMISSION GUIDELINES

FLUID CYTOLOGY

For fluids (effusions, BAL’s, synovial fluid): 

  • separate fluid into a red top tube and a purple top (EDTA) tube

  • submit a direct smear along with the fluid

  • if the sample volume is low and the fluid is NOT hemorrhagic, use a red top tube (important for culture, if recommended)

  • if the fluid is hemorrhagic, use a purple top (EDTA) tube, and run a PCV in clinic (and include the result on the submission form) - this helps determine if there is true hemorrhage contributing to the effusion

BONE MARROW CYTOLOGY

Submit up to 10 direct smears, and any left over bone marrow fluid in a purple top (EDTA) tube in case we need to prepare additional slides.

Include a thorough history and duration of hematology abnormalities.

Ideally, submit CBC results from the same day the bone marrow aspirate was obtained (results may be emailed separately once available).

FINE NEEDLE ASPIRATES AND IMPRESSION SMEARS

After making the smears, air dry.

Do not heat fix.

Do not stain (if you want to stain one or two with Diff-Quik in house, feel free to do so, and still send those along as well).

Check out the continuing education page for a more detailed guide to cytology sample collection and slide preparation by Dr. Craig.